ÿþ<HTML> <body bgcolor=white> <blockquote> <basefont color=#ff0000> <p align="center"> <A href="http://koko.gov.my"><img border="0" src="LOGO3.gif" width="100" height="100"> <A href="CCBR3.html"><img border="0" src="banerinm5.jpg" width="90" height="100"> <font face="Arial" color=green size="-1"> <h4><Marquee>PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY</Marquee> </h4> <center> <A href="index.htm"><font face="Arial" color=#0000FF size="-1">Home</FONT></A> | <A href="Profile.html"><font face="Arial" color=#0000FF size="-1">Profile</FONT></A> | <A href="CCBR3.html"><font face="Arial" color=#0000FF size="-1">Achievement</FONT></A> | <A href="Research.html"><font face="Arial" color=#0000FF size="-1">Programmes</FONT></A> | <A href="Program.html"><font face="Arial" color=#0000FF size="-1">Projects</FONT></A> | <A href="Staff.html"><font face="Arial" color=#0000FF size="-1">Staffs</FONT></A> | <A href="Publication.html"><font face="Arial" color=#0000FF size="-1">Publications</FONT></A> | <A href="Journals.html"><font face="Arial" color=#0000FF size="-1">Journals</FONT></A> |<br> <A href="Glossary.html"><font face="Arial" color=#0000FF size="-1">Biotech Glossary</FONT></A> | <A href="Bioinformatics.html"><font face="Arial" color=#0000FF size="-1">Bioinformatics</FONT></A> | <A href="Protocol1.html"><font face="Arial" color=#0000FF size="-1">Lab Protocol</FONT></A> | <A href="Notes.html"><font face="Arial" color=#0000FF size="-1">Notes</FONT></A> | <A href="University.html"><font face="Arial" color=#0000FF size="-1">Malaysia University</FONT></A> | <p> </TD></TR></TABLE><p> </center> <font size=2> <font color="black"> <center> <A href="Protocol1.html"><font face="Arial" color=BLACK size=3>MOLECULAR BIOLOGY: WORKING WITH DNA</FONT></A><p> <A href="Cloning.html"><font face="Arial" color=#0000FF size=3>CLONING</FONT></A><p> </CENTER> <font size=2> 1. <a href="Cloning1.html"><font face="Arial" color=#0000FF size="-1">3' Rapid Amplification of cDNA Ends (RACE) by PCR </FONT></A><br> 2. <a href="Cloning2.html"><font face="Arial" color=#0000FF size="-1">DNA Ligation </FONT></A><br> 3. <a href="Cloning3.html"><font face="Arial" color=#0000FF size="-1">Double-Stranded cDNA Synthesis for Cloning</FONT></A><br> 4. <a href="Cloning4.html"><font face="Arial" color=#0000FF size="-1">End-Filling DNA with Taq Polymerase </FONT></A><br> 5. <a href="Cloning5.html"><font face="Arial" color=#0000FF size="-1">Filling 5' Extensions to Create Flush Termini for Cloning (Klenow Reaction)</FONT></A><br> 6. <a href="Cloning6.html"><font face="Arial" color=#0000FF size="-1">Generation of a Probe for cDNA Cloning by PCR Using Peptide Sequence Information </FONT></A><br> 7. <a href="Cloning7.html"><font face="Arial" color=#0000FF size="-1">Labeling Oligonucleotides Using T4 Polynucleotide Kinase </FONT></A><br> 8. <a href="Cloning8.html"><font face="Arial" color=#0000FF size="-1">Ligation of Phosphorylated Linkers to Blunt-Ended Target Fragments </FONT></A><br> 9. <a href="Cloning9.html"><font face="Arial" color=#0000FF size="-1">M13 Library Construction from BAC or Bacteriophage P1 Clones </FONT></A><br> 10. <a href="Cloning10.html"><font face="Arial" color=#0000FF size="-1">Nested Deletions Using Exonuclease III and Mung Bean Nuclease </FONT></A><br> 11. <a href="Cloning11.html"><font face="Arial" color=#0000FF size="-1">Plasmid Rescue from P[lacZ] Lines in Drosophila </FONT></A><br> 12. <a href="Cloning12.html"><font face="Arial" color=#0000FF size="-1">Subcloning DNA Fragments into Plasmids - Ligation with T4 DNA Ligase</FONT></A><br> 13. <a href="Cloning13.html"><font face="Arial" color=#0000FF size="-1">Trimming 3' Extensions to Create Flush Termini for Cloning (T4 DNA Polymerase Reaction) </FONT></A><br> <p><br><P><BR><P><BR><P><BR><P><BR><P><BR><P><BR><HR> <center><font size=1><i>Cited in http://www.bio.com/protocolstools/browsdesc.jhtml