ÿþ<HTML> <body bgcolor=white> <blockquote> <basefont color=#ff0000> <p align="center"> <A href="http://koko.gov.my"><img border="0" src="LOGO3.gif" width="100" height="100"> <A href="CCBR3.html"><img border="0" src="banerinm5.jpg" width="90" height="100"> <font face="Arial" color=green size="-1"> <h4><Marquee>PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY</Marquee> </h4> <center> <A href="index.htm"><font face="Arial" color=#0000FF size="-1">Home</FONT></A> | <A href="Profile.html"><font face="Arial" color=#0000FF size="-1">Profile</FONT></A> | <A href="CCBR3.html"><font face="Arial" color=#0000FF size="-1">Achievement</FONT></A> | <A href="Research.html"><font face="Arial" color=#0000FF size="-1">Programmes</FONT></A> | <A href="Program.html"><font face="Arial" color=#0000FF size="-1">Projects</FONT></A> | <A href="Staff.html"><font face="Arial" color=#0000FF size="-1">Staffs</FONT></A> | <A href="Publication.html"><font face="Arial" color=#0000FF size="-1">Publications</FONT></A> | <A href="Journals.html"><font face="Arial" color=#0000FF size="-1">Journals</FONT></A> |<br> <A href="Glossary.html"><font face="Arial" color=#0000FF size="-1">Biotech Glossary</FONT></A> | <A href="Bioinformatics.html"><font face="Arial" color=#0000FF size="-1">Bioinformatics</FONT></A> | <A href="Protocol1.html"><font face="Arial" color=#0000FF size="-1">Lab Protocol</FONT></A> | <A href="Notes.html"><font face="Arial" color=#0000FF size="-1">Notes</FONT></A> | <A href="University.html"><font face="Arial" color=#0000FF size="-1">Malaysia University</FONT></A> | <p> </TD></TR></TABLE><p> </center> <font size=2> <font color="black"> <center> <A href="Protocol1.html"><font face="Arial" color=BLACK size=3>MOLECULAR BIOLOGY: WORKING WITH DNA</FONT></A><p> <A href="DNA Cells.html"><font face="Arial" color=#0000FF size=3>INTRODUCTION OF DNA INTO CELLS</FONT></A><p> </CENTER> <font size=2> 1. <a href="DNA Cells1.html"><font face="Arial" color=#0000FF size="-1">96-Well Yeast Transformation Protocol </FONT></A><br> 2. <a href="DNA Cells2.html"><font face="Arial" color=#0000FF size="-1">Amplification of Baculovirus Plaques and Preparation of High Titer Virus Stocks </FONT></A><br> 3. <a href="DNA Cells3.html"><font face="Arial" color=#0000FF size="-1">Baculovirus Transfection Using Calcium Chloride </FONT></A><br> 4. <a href="DNA Cells4.html"><font face="Arial" color=#0000FF size="-1">Calcium Phosphate Transformation of Mammalian Cells </FONT></A><br> 5. <a href="DNA Cells5.html"><font face="Arial" color=#0000FF size="-1">DNA Transfection of Eukaryotic Cells Using Calcium Phosphate </FONT></A><br> 6. <a href="DNA Cells6.html"><font face="Arial" color=#0000FF size="-1">Drosophila Tissue Culture Cell Transfection </FONT></A><br> 7. <a href="DNA Cells7.html"><font face="Arial" color=#0000FF size="-1">Electroporation of Competent E. coli TG1 Cells with Antibody Display Phagemid DNA</FONT></A><br> 8. <a href="DNA Cells8.html"><font face="Arial" color=#0000FF size="-1">Electroporation of COS-7 Cells </FONT></A><br> 9. <a href="DNA Cells9.html"><font face="Arial" color=#0000FF size="-1">Electroporation of Human Myeloid Cells (U937 and HL-60)</FONT></A><br> 10. <a href="DNA Cells10.html"><font face="Arial" color=#0000FF size="-1">Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants</FONT></A><br> 11. <a href="DNA Cells11.html"><font face="Arial" color=#0000FF size="-1">Hanahan Method of Transformation for High Efficiency Competent Cells </FONT></A><br> 12. <a href="DNA Cells12.html"><font face="Arial" color=#0000FF size="-1">Hanahan Transformation of E. coli </FONT></A><br> 13. <a href="DNA Cells13.html"><font face="Arial" color=#0000FF size="-1">High Efficiency Transformation of Yeast Cells </FONT></A><br> 14. <a href="DNA Cells14.html"><font face="Arial" color=#0000FF size="-1">High Efficiency Yeast Transformation Requiring Maintenance of a Previously Introduced Plasmid for Two-Hybrid Screens </FONT></A><br> 15. <a href="DNA Cells15.html"><font face="Arial" color=#0000FF size="-1">High-Efficiency Electro-Transformation of <I>E. coli</I></FONT></A><br> 16. <a href="DNA Cells16.html"><font face="Arial" color=#0000FF size="-1">Infection of Cells with Retroviruses </FONT></A><br> 17. <a href="DNA Cells17.html"><font face="Arial" color=#0000FF size="-1">Infection of Chicken Secondary Tissue Culture Cells </FONT></A><br> 18. <a href="DNA Cells18.html"><font face="Arial" color=#0000FF size="-1">Inoue Super Competent E. coli Preparation </FONT></A><br> 19. <a href="DNA Cells19.html"><font face="Arial" color=#0000FF size="-1">Lipofection of PC12 Cells </FONT></A><br> 20. <a href="DNA Cells20.html"><font face="Arial" color=#0000FF size="-1">Lithium Acetate Yeast Cell Transformation Using a Heat Shock Step </FONT></A><br> 21. <a href="DNA Cells21.html"><font face="Arial" color=#0000FF size="-1">PEG Precipitation for Making Transforming Quality DNA For Mammalian Cells </FONT></A><br> 22. <a href="DNA Cells22.html"><font face="Arial" color=#0000FF size="-1">PEG/DMSO Production of Competent <I>E. coli </I></FONT></A><br> 23. <a href="DNA Cells23.html"><font face="Arial" color=#0000FF size="-1">Preparation of Competent <I>E. coli</I> Cells using Calcium Chloride</FONT></A><br> 24. <a href="DNA Cells24.html"><font face="Arial" color=#0000FF size="-1">Preparation of Competent Yeast Cells with Lithium Acetate for Transformation </FONT></A><br> 25. <a href="DNA Cells25.html"><font face="Arial" color=#0000FF size="-1">Preparation of Electrocompetent Cells and Electro-Transformation of <I>E. coli</I></FONT></A><br> 26. <a href="DNA Cells26.html"><font face="Arial" color=#0000FF size="-1">Preparation of Electroporation-Competent Bacteria (Strain <I>E. coli</I> TG1) for Phage Antibody Libraries</FONT></A><br> 27. <a href="DNA Cells27.html"><font face="Arial" color=#0000FF size="-1">Preparation of Single-Stranded DNA for Site-Directed Mutagenesis </FONT></A><br> 28. <a href="DNA Cells28.html"><font face="Arial" color=#0000FF size="-1">Preparing and Using Competent Bacterial Cells for Electroporation </FONT></A><br> 29. <a href="DNA Cells29.html"><font face="Arial" color=#0000FF size="-1">Preparing Competent Yeast Cells for Frozen Storage and for Transformation </FONT></A><br> 30. <a href="DNA Cells30.html"><font face="Arial" color=#0000FF size="-1">Procedure for Transient or Stable Transfection of Adherent Cells using Lipofectamine </FONT></A><br> 31. <a href="DNA Cells31.html"><font face="Arial" color=#0000FF size="-1">Rapid and Simple Method of Yeast Cell Transformation </FONT></A><br> 32. <a href="DNA Cells32.html"><font face="Arial" color=#0000FF size="-1">Rapid Yeast Transformation </FONT></A><br> 33. <a href="DNA Cells33.html"><font face="Arial" color=#0000FF size="-1">Simple Plate Transformation of Yeast </FONT></A><br> 34. <a href="DNA Cells34.html"><font face="Arial" color=#0000FF size="-1">Transformation of Arabidopsis thalina by Vacuum Infiltration</FONT></A><br> 35. <a href="DNA Cells35.html"><font face="Arial" color=#0000FF size="-1">Transformation of Competent Yeast Prepared with Lithium Acetate </FONT></A><br> 36. <a href="DNA Cells36.html"><font face="Arial" color=#0000FF size="-1">Transformation of SK1 Yeast by Electroporation </FONT></A><br> 37. <a href="DNA Cells37.html"><font face="Arial" color=#0000FF size="-1">Transformation of Yeast Cells Using Lithium Acetate </FONT></A><br> 38. <a href="DNA Cells38.html"><font face="Arial" color=#0000FF size="-1">Transient Transfection and Spin Infection Protocol </FONT></A><br> 39. <a href="DNA Cells39.html"><font face="Arial" color=#0000FF size="-1">Transient Transfection of COS Cells </FONT></A><br> 40. <a href="DNA Cells40.html"><font face="Arial" color=#0000FF size="-1">Ultra Simple Yeast Transformation </FONT></A><br> <p><HR> <center><font size=1><i>Cited in http://www.bio.com/protocolstools/browsdesc.jhtml