ÿþ<HTML> <body bgcolor=white> <blockquote> <basefont color=#ff0000> <p align="center"> <A href="http://koko.gov.my"><img border="0" src="LOGO3.gif" width="100" height="100"> <A href="CCBR3.html"><img border="0" src="banerinm5.jpg" width="90" height="100"> <font face="Arial" color=green size="-1"> <h4><Marquee>PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY</Marquee> </h4> <center> <A href="index.htm"><font face="Arial" color=#0000FF size="-1">Home</FONT></A> | <A href="Profile.html"><font face="Arial" color=#0000FF size="-1">Profile</FONT></A> | <A href="CCBR3.html"><font face="Arial" color=#0000FF size="-1">Achievement</FONT></A> | <A href="Research.html"><font face="Arial" color=#0000FF size="-1">Programmes</FONT></A> | <A href="Program.html"><font face="Arial" color=#0000FF size="-1">Projects</FONT></A> | <A href="Staff.html"><font face="Arial" color=#0000FF size="-1">Staffs</FONT></A> | <A href="Publication.html"><font face="Arial" color=#0000FF 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size="-1"><i>E. coli</i> Alkaline Phosphatase Assay </FONT></A><br> 2. <a href="Enzymatic2.html"><font face="Arial" color=#0000FF size="-1"><i>Nco</i> I and <i>Not</i> I Restriction Digestion of the scFv Gene Repertoires </FONT></A><br> 3. <a href="Enzymatic3.html"><font face="Arial" color=#0000FF size="-1">Amino-allyl Reverse Transcription </FONT></A><br> 4. <a href="Enzymatic4.html"><font face="Arial" color=#0000FF size="-1">Dephosphorylation of DNA Using Calf Intestine Alkaline Phosphatase</FONT></A><br> 5. <a href="Enzymatic5.html"><font face="Arial" color=#0000FF size="-1">DNA Ligation </FONT></A><br> 6. <a href="Enzymatic6.html"><font face="Arial" color=#0000FF size="-1">End-Filling DNA with Taq Polymerase</FONT></A><br> 7. <a href="Enzymatic7.html"><font face="Arial" color=#0000FF size="-1">Extended Sequencing with Klenow Fragment </FONT></A><br> 8. <a href="Enzymatic8.html"><font face="Arial" color=#0000FF size="-1">Extended Sequencing with Klenow Fragment Using Double-Stranded DNA as Template</FONT></A><br> 9. <a href="Enzymatic9.html"><font face="Arial" color=#0000FF size="-1">Filling 5' Extensions to Create Flush Termini for Cloning (Klenow Reaction) </FONT></A><br> 10. <a href="Enzymatic10.html"><font face="Arial" color=#0000FF size="-1">General Protocol: Restriction Enzyme Digest of DNA </FONT></A><br> 11. <a href="Enzymatic11.html"><font face="Arial" color=#0000FF size="-1">Labeling of DNA Probes using Primer Extension </FONT></A><br> 12. <a href="Enzymatic12.html"><font face="Arial" color=#0000FF size="-1">Labeling Oligonucleotides </FONT></A><br> 13. <a href="Enzymatic13.html"><font face="Arial" color=#0000FF size="-1">Labeling Oligonucleotides Using T4 Polynucleotide Kinase</FONT></A><br> 14. <a href="Enzymatic14.html"><font face="Arial" color=#0000FF size="-1">Ligation in Low-Melting Temperature Agarose Gel </FONT></A><br> 15. <a href="Enzymatic15.html"><font face="Arial" color=#0000FF size="-1">Ligation of Phosphorylated Linkers to Blunt-Ended Target Fragments</FONT></A><br> 16. <a href="Enzymatic16.html"><font face="Arial" color=#0000FF size="-1">Nested Deletions Using Exonuclease III and Mung Bean Nuclease </FONT></A><br> 17. <a href="Enzymatic17.html"><font face="Arial" color=#0000FF size="-1">Nick Translation Labeling of DNA with ³-[32P]-dNTP </FONT></A><br> 18. <a href="Enzymatic18.html"><font face="Arial" color=#0000FF size="-1">Partial Restriction Digest of DNA </FONT></A><br> 19. <a href="Enzymatic19.html"><font face="Arial" color=#0000FF size="-1">Preparation and Purification of Taq Polymerase </FONT></A><br> 20. <a href="Enzymatic20.html"><font face="Arial" color=#0000FF size="-1">Preparation of Topoisomerase I from Drosophila Embryos </FONT></A><br> 21. <a href="Enzymatic21.html"><font face="Arial" color=#0000FF size="-1">Radiolabeling of Oligonucleotides </FONT></A><br> 22. <a href="Enzymatic22.html"><font face="Arial" color=#0000FF size="-1">Reverse Transcriptase Assay Optimized for Avian Sarcoma Virus</FONT></A><br> 23. <a href="Enzymatic23.html"><font face="Arial" color=#0000FF size="-1">Reverse Transcriptase Assay Optimized for Molonoy Murine Leukemia Virus (MuLV)</FONT></A><br> 24. <a href="Enzymatic24.html"><font face="Arial" color=#0000FF size="-1">Screening Genomic or cDNA Libraries with Radiolabeled DNA Probes </FONT></A><br> 25. <a href="Enzymatic25.html"><font face="Arial" color=#0000FF size="-1">Subcloning DNA Fragments into Plasmids - Ligation with T4 DNA Ligase </FONT></A><br> 26. <a href="Enzymatic26.html"><font face="Arial" color=#0000FF size="-1">The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol using ±-Phosphothioate Nucleotide Analogs </FONT></A><br> 27. <a href="Enzymatic27.html"><font face="Arial" color=#0000FF size="-1">The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol </FONT></A><br> 28. <a href="Enzymatic28.html"><font face="Arial" color=#0000FF size="-1">Trimming 3' Extensions to Create Flush Termini for Cloning (T4 DNA Polymerase Reaction) </FONT></A><br> <p><HR> <center><font size=1><i>Cited in http://www.bio.com/protocolstools/browsdesc.jhtml