ÿþ<HTML> <body bgcolor=white> <blockquote> <basefont color=#ff0000> <p align="center"> <A href="http://koko.gov.my"><img border="0" src="LOGO3.gif" width="100" height="100"> <A href="CCBR3.html"><img border="0" src="banerinm5.jpg" width="90" height="100"> <font face="Arial" color=green size="-1"> <h4><Marquee>PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY</Marquee> </h4> <center> <A href="index.htm"><font face="Arial" color=#0000FF size="-1">Home</FONT></A> | <A href="Profile.html"><font face="Arial" color=#0000FF size="-1">Profile</FONT></A> | <A href="CCBR3.html"><font face="Arial" color=#0000FF size="-1">Achievement</FONT></A> | <A href="Research.html"><font face="Arial" color=#0000FF size="-1">Programmes</FONT></A> | <A href="Program.html"><font face="Arial" color=#0000FF size="-1">Projects</FONT></A> | <A href="Staff.html"><font face="Arial" color=#0000FF size="-1">Staffs</FONT></A> | <A href="Publication.html"><font face="Arial" color=#0000FF 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size="-1">Complementary Oligonucleotides and Nested Deletions for Linker Scanning </FONT></A><br> 2. <a href="Mutagenesis2.html"><font face="Arial" color=#0000FF size="-1">Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants </FONT></A><br> 3. <a href="Mutagenesis3.html"><font face="Arial" color=#0000FF size="-1">EMS (Ethylmethane Sulfonate) Mutagenesis of Yeast #1 </FONT></A><br> 4. <a href="Mutagenesis4.html"><font face="Arial" color=#0000FF size="-1">EMS Mutagenesis of Yeast #2 </FONT></A><br> 5. <a href="Mutagenesis5.html"><font face="Arial" color=#0000FF size="-1">Ethylmethane Sulfonate (EMS) Mutagenesis of <i>Arabidopsis</i></FONT></A><br> 6. <a href="Mutagenesis6.html"><font face="Arial" color=#0000FF size="-1">Generation of Uni-Directional Nested Deletions in Plasmid DNA </FONT></A><br> 7. <a href="Mutagenesis7.html"><font face="Arial" color=#0000FF size="-1">Hydroxylamine Mutagenesis of Plasmid DNA </FONT></A><br> 8. <a href="Mutagenesis8.html"><font face="Arial" color=#0000FF size="-1"><i>In Vitro</i> Mutagenesis with <i>E. coli</i> dut ung Single Stranded DNA</FONT></A><br> 9. <a href="Mutagenesis9.html"><font face="Arial" color=#0000FF size="-1">Kunkel Method of Site-Directed Mutagenesis</FONT></A><br> 10. <a href="Mutagenesis10.html"><font face="Arial" color=#0000FF size="-1">Oligonucleotide-Directed Mutagenesis </FONT></A><br> 11. <a href="Mutagenesis11.html"><font face="Arial" color=#0000FF size="-1">Plasmid Rescue from P[lacZ] Lines in <i>Drosophila </i></FONT></A><br> 12. <a href="Mutagenesis12.html"><font face="Arial" color=#0000FF size="-1">Preparation of Single-Stranded DNA for Site-Directed Mutagenesis</FONT></A><br> 13. <a href="Mutagenesis13.html"><font face="Arial" color=#0000FF size="-1">Production of Uracil-Containing Single Stranded DNA (ssDNA) Template</FONT></A><br> 14. <a href="Mutagenesis14.html"><font face="Arial" color=#0000FF size="-1">Random Mutagenesis by PCR </FONT></A><br> 15. <a href="Mutagenesis15.html"><font face="Arial" color=#0000FF size="-1">Removal of Selectable Marker Cassettes from Transfected ES clones using Cre-Lox Mediated Recombination</FONT></A><br> 16. <a href="Mutagenesis16.html"><font face="Arial" color=#0000FF size="-1">Site-Directed Mutagenesis of Double Stranded DNA by Elimination of a Unique Restriction Enzyme Site </FONT></A><br> 17. <a href="Mutagenesis17.html"><font face="Arial" color=#0000FF size="-1">Site-Directed Mutagenesis Using a Single-Stranded DNA Template </FONT></A><br> 18. <a href="Mutagenesis18.html"><font face="Arial" color=#0000FF size="-1">Synthesis of Specific Sequence Using Mutually Priming Long Oligonucleotides </FONT></A><br> 19. <a href="Mutagenesis19.html"><font face="Arial" color=#0000FF size="-1">The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol using ±-Phosphothioate Nucleotide Analogs</FONT></A><br> 20. <a href="Mutagenesis20.html"><font face="Arial" color=#0000FF size="-1">The Incremental Truncation for the Creation of Hybrid EnzYmes (ITCHY) Protocol </FONT></A><br> 21. <a href="Mutagenesis21.html"><font face="Arial" color=#0000FF size="-1">X-Ray Mutagenesis of <i>Drosophila</i> </FONT></A><br> <p><HR> <center><font size=1><i>Cited in http://www.bio.com/protocolstools/browsdesc.jhtml