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A. Cleavage of Glutamic Acid-X (Glu-X) Bonds (Cleavage Will Not Occur if X=Proline)
1. Dissolve the protein substrate in 100 mM Ammonium Bicarbonate at 10 mg/ml.
2. Add 0.03 volumes of the enzyme to the protein substrate and incubate for 2 to 18 hours (determine empirically) at 37°C.
B. Cleavage of Glutamic Acid-X (Glu-X) and Aspartic Acid-X (Asp-X) Bonds (Cleavage Will Not Occur if X=Proline)
1. Dissolve the protein substrate in 100 mM Phosphate Buffer at 10 mg/ml.
2. Add 0.03 volumes of the enzyme to the protein substrate and incubate for 2 to 8 hours (determine empirically) at 37°C.
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| 100 mM Ammonium Bicarbonate |
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| Endoproteinase Glutamic Acid-C |
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| 100 mM Phosphate Buffer, pH 7.8 |
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Sodium Phosphate
Endoproteinase Glutamic Acid-C
Ammonium Bicarbonate
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No hints are associated with this bioProtocol
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Tomasselli, AG, Frank R, Schiltz. The complete primary structure of GTP:AMP phosphotransferase from beef heart. FEBS Lett. 1986, 202:303-307.
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